By Simon P. Langdon
Specialist researchers describe intimately their best and up to date tools for becoming melanoma cells within the laboratory. there are ways to symbolize and authenticate telephone traces, to isolate and advance particular varieties of melanoma cells, and to improve new mobilephone line versions. useful assays are supplied to judge clonogenicity, mobile proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and mobilephone cycle parameters. different tools allow the amendment of melanoma cells for transfection, improvement of drug resistance, immortalization, and move in vivo; the co-culture of other cellphone kinds; and the detection and remedy of illness.
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Extra info for Cancer Cell Culture. Methods and Protocols
R. , Hay, R. , Drexler, H. , Macleod, R. A. , and Freshney, R. I. (2000) Cell contamination leads to inaccurate data: we must take action now. Nature 403, 356. 3. Macleod, R. A. , Dirks, W. , and Drexler, H. G. (2002) Persistent use of misidentified cell lines and its prevention. Genes Chromosomes Cancer 33, 103–105. 4. Gartler, S. M. (1968) Apparent HeLa contamination of human heterodiploid cell lines. Nature 217, 750–751. 5. Nelson-Rees, W. , Flandermeyer, R. , and Hawthorne P. K. (1974) Banded marker chromosomes as indicators of intraspecies cellular contamination.
Science 195, 1343–1344. 9. Gey, G. , Coffman, W. , and Kubicek, M. T. (1952) Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium. Cancer Res. 12, 264–265. Characterization of Cell Lines 41 10. MacLeod, R. A. , Dirks, W. , and Drexler, H. G. (1999) Widespread intraspecies cross-contamination of human tumor cell lines arising at source. Int. J. Cancer 83, 555–563. 11. UKCCCR (2000) UKCCCR guidelines for the use of cell lines in cancer research. Brit. J.
3. Expression Profiles Certain proteins can be particularly informative in helping to characterize cells and their origins, and these can be measured by a wide variety of techniques such as Western blotting, FACS analysis, or immunocytochemistry. Intermediate filaments are particularly helpful, including cytokerations (epithelial cells), vimentin (mesenchymal cells), desmin ( myogenic cells), neurofilament protein (neurons), and glial fibrillar 40 Langdon acidic protein (glial cells) (39,40). In addition to the above more general proteins, many cell lines are strongly characterized by the presence of particular proteins.