By Simon P. Langdon

Specialist researchers describe intimately their best and up to date tools for becoming melanoma cells within the laboratory. there are ways to symbolize and authenticate telephone traces, to isolate and advance particular varieties of melanoma cells, and to improve new mobilephone line versions. useful assays are supplied to judge clonogenicity, mobile proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and mobilephone cycle parameters. different tools allow the amendment of melanoma cells for transfection, improvement of drug resistance, immortalization, and move in vivo; the co-culture of other cellphone kinds; and the detection and remedy of illness.

Show description

Read Online or Download Cancer Cell Culture. Methods and Protocols PDF

Best oncology books

Evaluating Alternative Cancer Therapies: A Guide to the Science and Politics of an Emerging Medical Field

In comparing replacement melanoma treatments, David Hess has interviewed the key opinion leaders within the substitute melanoma remedy box - clinicians, researchers, sufferer advocacy leaders, and reporters - who clarify their philosophy of assessment, their healing personal tastes, and the political and financial hurdles to getting the required learn performed.

Malignant Melanoma - A Medical Dictionary, Bibliography, and Annotated Research Guide to Internet References

This can be a 3-in-1 reference e-book. It provides a whole scientific dictionary masking thousands of phrases and expressions in relation to malignant cancer. It additionally offers huge lists of bibliographic citations. eventually, it offers info to clients on the best way to replace their wisdom utilizing a number of net assets.

Palliative Care in Oncology

Palliative care presents complete aid for seriously affected sufferers with any life-limiting or life-threatening prognosis. to do that successfully, it calls for a disease-specific technique because the sufferers’ wishes and scientific context will range reckoning on the underlying prognosis. specialists within the box of palliative care and oncology describe intimately the desires of sufferers with complicated melanoma compared to people with non-cancer affliction and likewise determine the necessities of sufferers with diverse melanoma entities.

100 questions and answers about bipolar (manic-depressive) disorder

Offers doctor's and patient's perspectives of bipolar disease. summary: the one quantity to supply the doctor's and patient's view and offers you authoritative, functional solutions for your questions on remedies, coping techniques. learn extra. ..

Extra info for Cancer Cell Culture. Methods and Protocols

Sample text

R. , Hay, R. , Drexler, H. , Macleod, R. A. , and Freshney, R. I. (2000) Cell contamination leads to inaccurate data: we must take action now. Nature 403, 356. 3. Macleod, R. A. , Dirks, W. , and Drexler, H. G. (2002) Persistent use of misidentified cell lines and its prevention. Genes Chromosomes Cancer 33, 103–105. 4. Gartler, S. M. (1968) Apparent HeLa contamination of human heterodiploid cell lines. Nature 217, 750–751. 5. Nelson-Rees, W. , Flandermeyer, R. , and Hawthorne P. K. (1974) Banded marker chromosomes as indicators of intraspecies cellular contamination.

Science 195, 1343–1344. 9. Gey, G. , Coffman, W. , and Kubicek, M. T. (1952) Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium. Cancer Res. 12, 264–265. Characterization of Cell Lines 41 10. MacLeod, R. A. , Dirks, W. , and Drexler, H. G. (1999) Widespread intraspecies cross-contamination of human tumor cell lines arising at source. Int. J. Cancer 83, 555–563. 11. UKCCCR (2000) UKCCCR guidelines for the use of cell lines in cancer research. Brit. J.

3. Expression Profiles Certain proteins can be particularly informative in helping to characterize cells and their origins, and these can be measured by a wide variety of techniques such as Western blotting, FACS analysis, or immunocytochemistry. Intermediate filaments are particularly helpful, including cytokerations (epithelial cells), vimentin (mesenchymal cells), desmin ( myogenic cells), neurofilament protein (neurons), and glial fibrillar 40 Langdon acidic protein (glial cells) (39,40). In addition to the above more general proteins, many cell lines are strongly characterized by the presence of particular proteins.

Download PDF sample

Rated 4.14 of 5 – based on 13 votes