By Nils Metzler-Nolte

This booklet offers a different advent into the sphere of bioinorganic chemistry via functional laboratory experiments. issues comprise many facets of recent bioinorganic chemistry equivalent to version structures for metalloenzymes, biosensors, steel bioconjugates and metal-based medicinal drugs. each one bankruptcy encompasses a short creation, through unique experimental techniques, accomplished with all helpful historical past info for the coed in addition to their teachers. A invaluable complement to plain textbooks of inorganic and bioinorganic chemistry crucial for all teachers instructing laboratory classes in most cases and inorganic chemistry

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Rev. 2006, 86, 583–650. [11] T. R. Johnson, B. E. Mann, J. E. Clark, R. Foresti, C. J. Green, R. , Angew. Chem. Int. Ed. 2003, 42, 3722–3729. [12] S. P. L. Cary, J. A. Winger, E. R. Derbyshire, M. A. Marletta, Nitric oxide signaling: No longer simply on or off, Trends Biochem. Sci 2006, 31, 231–239. [13] S. Aono, Metal-containing sensor proteins sensing diatomic gas molecules, Dalton Trans. 2008, 3137–3146. 30 3 Carbon monoxide and nitric oxide as small molecule messengers [14] E. M. Boon, M. A.

In the experiments described in this chapter, we will instead use UV/Vis spectroscopy as the detection method. The characteristic spectral changes in the Soret- and Q-band region of heme proteins upon binding of carbon monoxide and nitric oxide can be used to follow the time course of the reaction and also to quantify the amount of CO and NO released. This system also constitutes a very simple example of a protein-based assay system and will, in addition, introduce you to some important properties of heme proteins and their small molecule adducts.

Record a UV/Vis spectrum in the 200 to 800 nm range. Leave both cuvettes in the spectrometer and add to the sample cuvette 5 l of solution B and to the reference cuvette 5 l of solution C. Carefully mix with a small glass rod and let equilibrate for 10 min. Then record another UV/Vis spectrum. Repeat the sequence of addition of solutions B and C, mixing, equilibration, and measurement of UV/Vis spectra until three consecutive measurements do not show any spectral changes or the complete 100 l of solutions B and C have been added.

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