By B.T. Kang, A.N. Atta-Krah, L. Reynolds

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14). The convex surfaces of both lenses face downward. Oculars in this group are sometimes spoken of as negative This type of ocular is made with which bends the pencils of light corning from the objective towards the axis without altering to any great* extent eyepieces. a large FIG. 14. EL, eye Huygens eyepiece. FL, field lens. fi, real image formed between the ocular lenses and the diaphragm d. (Redrawn from Gage, The Microscope, The Comstock lens. ) field lens, the convergence or divergence of the rays in the individual pencils.

Compensating eyepiece. EL, lens. eye FL, field lens of three comcomposed ponents. ri real image formed below the lenses at the diaphragm d. ) illuminating, witrT transmitted light, the object to be studied on the It is located under the stage of the microscope between nicroscope. lie mirror and the object, whereas the objective and ocular lenses are 'or ocated above the stage. lenser (Fig. 16). It is sometimes referred to as . con- FUNDAMENTAL PRINCIPLES OF BACTERIOLOGY 24 A condenser is necessary for the examination of an object with an oilimmersion objective in order to obtain adequate illumination.

Working Distance of Covered Objects. If the object is covered with a slip, the free distance from the upper surface of the cover slip to the front of the objective will be less than in the case of an uncovered object. It is obvious from this that if the cover glass is thicker than the working cover distance of the objective, it will be impossible to get the object in focus. other hand if the glass is thin, it will be possible to get the object On the in focus but the focus of the microscope different from that on an uncovered object.

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